Ez biotin quantitation kit from pierce biocompare product. Pierce biotin quantitation kit thermo fisher scientific. The endotoxin concentration in a sample is measured using the pierce lal chromogenic endotoxin quantitation kit via a chromogenic signal generated in the presence of endotoxins. Thermo scientific pierce biotin quantitation kit ez. The kit has a detection sensitivity limit of 1 x 109 gcml genome copyml for unpurified aav2 or aavdj supernatants, or 5 x 10 10 gcml for purified aav sample from any serotype, which is sufficient for mid or hightiter aav samples. The habaavidin premix is supplied in convenient thermo scientific noweigh microtube packaging, which eliminates the difficulties associated with weighing small quantities of reagent. Pierce biotin quantitation kit thermo fisher scientific the thermo scientific pierce biotin quantitation kit contains a premix of. The activated proenzyme then catalyzes the splitting of p. Biotin quantitation kit colorimetric k811 biovision. Pierce biotechnology po box 117 815 9680747 3747 n.
This colorimetric biotin quantitation kit provides a convenient method for estimating the molar ratio of biotin to protein in biotinprotein conjugates or for quantitating biotin concentration in a solution. They are all wellcharacterized, robust assays that provide consistent, reliable results. Protein quantitation kit is used for determining the concentration of protein in solution. Pierce offers seven colorimetric assays for detection and quantitation of total protein. The biotin 3 end dna labeling kit from pierce biotechnology incorporates biotinylated ribonucleotides onto the 3 end of dna strands by using terminal deoxynucleotidyl transferase tdt.
Many of the biotin labeling kits from pierce use an nhsbiotin reagent without the chromogenic fluorophore. Thermo scientific pierce biotin quantitation kit available on gsava contract for federal government customers only. Assay is suitable for measuring protein concentration in the range of 0. The thermo scientific biotin 3 end dna labeling kit uses tdt to incorporate biotinylated ribonucleotides onto the 3 end of dna strands. Pdf biotin switch assays for quantitation of reversible.
Biotin, a 244 dalton vitamin found in tiny amounts in all living cells, binds with high affinity to avidin, streptavidin and thermo scientific neutravidin biotinbinding protein. This kit can be used to determine accurately the labeling efficiency of biotin labeled molecules. This labeling strategy has the advantage of localizing the biotin to the 3 end of the probe where it is less likely to interfere with hybridization or sequencespecific binding of proteins. Thermo scientific pierce biotin quantitation kit includes aliquots of haba reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins. Both nucleic acid and protein quantitation can be performed in single tube or microplate assays. Direct streptavidinbinding assay does not accurately quantitate. The accublue high sensitivity assay is also available without any dna standards 31008, for those that wish to use their own standard. Thermo scientific pierce protein assay technical handbook. Pdf ingel detection of biotinprotein conjugates with a. Cell biolabs high sensitivity protein quantitation assay kit utilizes a fluorogenic detection dye to measure primary aliphatic amines peptides or proteins in various samples with much greater sensitivity than the conventional colorimetric protein determination methods. The thermo scientific pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Development of a sensitive, rapid, biotinstreptavidin. Ample materials are provided to perform 100 assays in cuvette format or 500 assays in a 96well format.
Fluoreporter biotin quantitation assay kit for biotinylated proteins f30751 figure 1. Application methods p a g e 1 080409 nucleic acid and protein quantitation methods prepared by. The thermo scientific pierce biotin quantitation kit contains a premix of haba and avidin and a biotinylated horseradish peroxidase hrp positive control. Thermo scientific pierce biotin quantitation kit includes aliquots of haba reagent. Ez biotin quantitation kit was purchased from pierce usa. The habaavidin premix is supplied in convenient noweigh microtube packaging, which eliminates the difficulties as sociated with weighing small quantities of reagent. The tdt prefers to label singlestranded dna, but can also label doublestranded dna with blunt or 3 overhangs, but at a lower efficiency. Table 1 comparison of common dna, rna and protein quantitation assays method detection. It isnt always easy to find a trusted source of lab equipment and chemicals like this this pierce lal chromogenic endotoxin quantitation kit. Piercea protein assay kit bc thermo fisher scientific. The thermo scientific ez biotin quantitation kit contains premeasured doses of the avidinhaba mixture and biotinylated horseradish peroxidase positive control to simplify reagent preparation and minimize the amount of waste generated. Urine solutions for the biotin doseresponse experiment were prepared by pooling two nonpregnant patient urine samples, resulting in a urine matrix with an hcg concentration pdf. In parallel, a set of known biotin concentrations was used to construct a standard.
Thermo scientific pierce immunostain enhancer 14 thermo scientific dapi stain 15 thermo scientific hoechst 33342 stain 1617 thermo scientific dylight conjugates 1819 licor odyssey instrument products 2021 fluorescence biotin quantitation kit 2223 fluorescent protein gel stains 24. Assessment of biotin interference with qualitative point. Enzymatic method for determining lamino acids lamino. Piercetm bca protein assay kit thermopierce, catalog number. Cell surface protein biotinylation and analysis bioprotocol. The assay employs haba 4hydroxyazobenzene2carboxylic acid, a reagent that shows dramatic spectral changes when bound to avidin. Ezlink sulfonhs biotin sulfosuccinimidyl6 biotin amidohexanoate, 25mg. Haba 24hydroxyazobenzene benzoic acid and avidin are the basis of the kit.
The thermo scientific pierce biotin quantitation kit contains a premix of. The protein quantification kit provides a simple and rapid procedure for determining the concentration of protein in solution. Conjugation of oligosaccharides to probes and surfaces is useful in the development of biochemical assays to assess carbohydrateprotein interactions. The assay employs haba 4hydroxyazobenzene2carboxylic acid, a reagent that shows dramatic. The thermo scientific pierce mass spec sample prep kit for cultured cells is an easytouse, comprehensive kit for preparation of clean peptide mixtures from cultured cells for ms analysis. Highly purified distilled and deionized water was used throughout. All samples and standards should be run in duplicate. Meridian road rockford, ll 61105 usa 815 9687316 fax number description. Samples can be measured on a microplate absorbance reader at 405nm. This colorimetric biotin quantitation kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin protein conjugates or for quantitating biotin concentration in a solution. The assay utilizes haba 24hydroxyphenylazobenzoic acid, a reagent that shows dramatic spectral changes when bound to avidin. The pierce biotin quantitation kit contains a premix of haba and avidin and a biotinylated horseradish peroxidase hrp positive control.
L of the habaavidin premix solution from step 2 to the cuvette. Urine specimens were stored at 28 c, protected from light, for no longer than 7 days prior to the measurement of biotin and creatinine concentration roche crej2, c701. Ingel detection of biotinprotein conjugates with a green fluorescent streptavidin probe. Biotin quantitation with quanttag kit from vector laboratories. The fluorescence biotin quantitation kit is requires only 10 l of sample. Biotin quantitation kit colorimetric k811 biovision, inc. Thermo scientific pierce fluorescence biotin quantitation kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled. Sensolyte biotin quantitation kit fluorimetric size. Biotin quantitation reagents and kits fisher scientific. The thermo scientific pierce lal chromogenic endotoxin quantitation kit measures the amount of endotoxin in a protein, peptide or antibody sample using the limulus amebocyte lysate lal assay. Amplite colorimetric biotin quantitation kit aat bioquest. Since biotin is a relatively small molecule, it can be conjugated to many proteins without significantly altering their biological activity. Proceed to the calculation of moles of biotin per mole of protein section. The assay is performed in a 96well microtiter plate format and measured within 15 minutes.
Biotin quantitation reagents and kits thermo scientific pierce biotin quantitation kit haba dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins. Our kit is more accurate than the other suppliers fluorescent biotin assay brand i. It consists of labeling cell surface proteins with a biotin reagent before lysing the cells, and isolating these tagged proteins by. Collectively, they represent the stateoftheart for colorimetric detection and quantitation of total protein. Procedure for quantitation of moles of biotin per mole of protein microplate format. A modified microbradford procedure for elimination of interference from sodium dodecyl sulfate, other detergents and. May 24, 2007 the biotin 3 end dna labeling kit from pierce biotechnology incorporates biotinylated ribonucleotides onto the 3 end of dna strands by using terminal deoxynucleotidyl transferase tdt. Biotin 3 end dna labeling kit from pierce biotechnology. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. For proteins of lower molecular weight or multiple biotin labels, less protein can be used.
Biotin vitamin h is an important biomolecule that has been widely used in biotinylation reactions to label antibodies or other proteins of interest. Using a chromogenic biotin reagent offers many advantagesyou can use simple uv quantitation to determine biotin incorporation with no need for the haba assay, an additional assay required to determine the extent of biotin incorporation. Biotin quantitation kit, quanttag vector laboratories, inc. The bca protein assay kit provides a simple, rapid, detergent tolerant up to 5% procedure for determining the concentration of proteins in solution.
Pdf quantitative recovery of biotinylated proteins from. Sulfosuccinimidyl6biotinamido hexanoate nhslcbiotin was purchased from pierce chemical rockford, il. The thermo scientific pierce lal chromogenic endotoxin quantitation kit is an efficient, quantitative endpoint assay for the detection of gramnegative bacterial endotoxins. Biovision biotin quantitation kit colorimetric, k811100. The convenient assay can be performed either in a cuvette or in a microplate. Assessment of biotin interference with qualitative pointof. The sensolyte biotin quantitation assay kit provides a convenient assay for estimating the molar ratio of biotin to protein in biotinprotein conjugates or for quantifying biotin concentration in a solution. Quicktiter aav quantitation kit provides an efficient system for rapid quantitation of aav titer for both viral supernatant and purified virus. Bacterial endotoxin catalyzes theactivation of a proenzyme in the modified limulus amebocyte lysate lal. Thermo scientific pierce biotin quantitation kit ez biotin. Fluorescence biotin quantitation kit thermo fisher scientific. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Dilutions must be accounted in the calculation step.
The pierce mass spec sample prep kit for cultured cells contains all the necessary reagents and enzymes to prepare up to 20 samples 1 million cells. An assay for biotin that uses europium coupled to streptavidin and. The accublue high sensitivity dsdna quantitation kit offers sensitive and selective detection of purified dsdna samples with a linear detection range from 0. Colorimetric biotin assay kit mak171 technical bulletin. Thermo scientific pierce lal chromogenic endotoxin. This kit can be used to determine accurately the labeling efficiency of biotinlabeled molecules.
Pdf the strong interaction between streptavidin and biotin is one of the most. The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. By contrast, the traditional colorimetric method requires 100 l of sample, and another leading suppliers fluorescent assay kit requires 50 l of sample. The thermo scientific fluorescence biotin quantitation kit requires only 10. The quanttag biotin kit bdk2000 is designed to determine the amount of free biotin in solution or the number of biotins attached to nucleic acids, proteins, or other macromolecules.
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